Blood physiology
Sylabus zajęć
Informacje podstawowe
Kierunek studiów
Biotechnology
Specjalność
-
Jednostka organizacyjna
Wydział Biologii
Poziom studiów
Studia drugiego stopnia
Forma studiów
Studia stacjonarne
Profil studiów
Profil ogólnoakademicki
Cykl dydaktyczny
2022-2025
Języki wykładowe
Angielski
Obligatoryjność
Fakultatywny
Blok zajęciowy
Przedmioty nieprzypisane
Prowadzący zajęcia
dr hab. Lucyna Mrówczyńska, lumro@amu.edu.pl
|
Okres
Semestr 4
|
Forma zajęć / liczba godzin / forma zaliczenia
|
Liczba punktów ECTS
3.0
|
Cele kształcenia dla zajęć
1. Provide knowledge about development of the hematopoietic system in ontogenesis.
2. Presenting dynamics of normal hematopoiesis and differnt types of bone marrow disorders.
3. Describe of objectives, types and risk of blood transfusions and hematopoetic stem cell transplantation.
4. Provide knowledge about haemorrhagic diarrhea and anemia.
5. Presenting the human red blood cell as a model for the study of toxic effects of biotic and abiotic factors under physiological and oxidative stress conditions.
6. Provide knowledge about drug delivery by red blood cells.
7. Providing knowledge about preservation and storage of blood and blood products, artificial blood and blood replacement.
8. Improving skills in work with human blood cells, confocal microscope, scanning electron microscope, flow cytometer (FACS).
9. Developing skills in planning experiments with blood components and blood data analysis.
Wymagania wstępne
cell biology, animal anatomy and physiology.Efekty uczenia się dla zajęć
| Symbol EU dla zajęć/przedmiotu | Po zakończeniu zajęć i potwierdzeniu osiągnięcia EU student/ka: | Symbole EK dla kierunku studiów |
| Efekt_01 | Describe development of the human hematopoietic system and characterize hematopoietic stem cells. | K_W01, K_K02 |
| Efekt_02 | Describe bone marrow structure in physiological and pathological conditions and present the causes of bone marrow disorders. | K_W04, K_K01 |
| Efekt_03 | List and compare different sources of hematopoietic stem cells for transplantation. | K_W05, K_U04 |
| Efekt_04 | Describe haemorrhagic diarrhea and anemia and list of reasons of shortening of erythrocyte life span. Compare physiological significance of neocytolysis and eryptosis. | K_W05, K_U04 |
| Efekt_05 | Work with human erythrocytes as a useful cell model in the study of natural and syntetic bioactive factors (including nanoaprticels) under physiological and oxidative stress conditions. | K_W09, K_U04, K_W04, K_K05, K_U07, K_U05 |
| Efekt_06 | Describe red blood cells as a natural drug transporters. | K_W05, K_W09, K_W11 |
| Efekt_07 | Know problems during conventional blood storage in blood banks and the effects of blood banking time on the critical parameters of erythrocytes. | K_W09, K_W04 |
| Efekt_08 | List and compare blood transfusion risks. | K_W05, K_W04 |
| Efekt_09 | Describe and compare artificial red blood cells and different types of blood substitutes. | K_W05, K_W09, K_K08, K_U04 |
| Efekt_10 | Compare normal and pathological bone marrows slides and blood smear, perform blood slides, analyse blood cells populations using confocal microscope, electron scanning microscope and flow cytometer. | K_W04, K_W08, K_W09, K_K01, K_K04 |
Treści programowe
| Lp. | Treści programowe dla zajęć/przedmiotu | Symbol EU dla zajęć/przedmiotu |
| 1. |
Prenatal and postnatal hematopoiesis - the properties and functions that define hematopoietic stem cells and hematopoetic niches. |
Efekt_01, Efekt_09 |
| 2. |
Bone marrow structure, types and functions in health and diseases (aplastic anemia, acute and chronic leukaemia, lymphoma, radiation and chemical injury, aging-induced alterations). |
Efekt_02, Efekt_01 |
| 3. |
Sources and classification of hematopoietic stem cells for transplantation: bone marrow, peripheral blood, mobilized peripheral blood and cord blood. |
Efekt_03, Efekt_01 |
| 4. |
Causes, types and symptoms of haemorrhagic diarrhea and anemia; modification of normal erythrocyte life span – neocytolysis, erytptosis, oxidative stress-induced haemolysis. |
Efekt_04, Efekt_07, Efekt_05 |
| 5. |
Erythrocytes as a useful cell model in the study of toxic effects of biotic and abiotic factors. |
Efekt_05, Efekt_07, Efekt_06 |
| 6. |
Application and safety of red blood cells as a novel drug delivery system. |
Efekt_06 |
| 7. |
Banking of blood problems – storage-related damage and morphological changes of red blood cells. |
Efekt_07, Efekt_05, Efekt_04 |
| 8. |
Blood transfusion-transmitted diseases. |
Efekt_08 |
| 9. |
Development of artificial red blood cells and blood substitute. |
Efekt_06, Efekt_07, Efekt_09 |
| 10. |
Analysis of intact and fixed blood cells, and different types of slides of bone marrow. |
Efekt_10 |
Informacje dodatkowe
| Metody i formy prowadzenia zajęć |
|---|
| Sposoby oceniania | Symbole EK dla modułu zajęć/przedmiotu | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| EK_1 | EK_2 | EK_3 | EK_4 | EK_5 | EK_6 | EK_7 | EK_8 | EK_9 | EK_10 | ||
| Kryteria oceniania wg skali stosowanej w UAM | |
|---|---|
| bardzo dobry (bdb; 5,0): | |
| dobry plus (+db; 4,5): | |
| dobry (db; 4,0): | |
| dostateczny plus (+dst; 3,5): | |
| dostateczny (dst; 3,0): | |
| niedostateczny (ndst; 2,0): | |
Literatura
Wydawnictwa książkowe
- 1. Rogers K.: Blood: physiology and circulation, Britannica Educational Publishing, New York, 2011
2. Bonifer C., Cockerill P.N. : Transcriptional and Epigenetic Mechanisms Regulating Normal and Aberrant Blood Cell Development, Springer , Heidelberg New York Dordrecht Lond, 2014
Artykuły w czasopismach
- 1. Lang E., Lang F. (2015): Mechanisms and pathophysiological significance of eryptosis, the suicidal erythrocyte death., Seminars in Cell & Developmental Biology, 39: 35–42
2. Pretorius E, Olumuyiwa-Akeredolu OO, Mbotwe S, Bester J. (2016): Erythrocytes and their role as health indicator: Using structure in a patient-orientated precision medicine approach., Blood Rev. , 30: 263-73
3. Villa CH, Seghatchian J, Muzykantov V. (2016): Drug delivery by erythrocytes: "Primum non nocere"., Transfus Apher Sci., 55: 275-80
4. Lux S.E (2016): Anatomy of the red cell membraneskeleton: unanswered questions., Blood, 127: 187-199
5. Kolb HJ. (2017): Hematopoietic stem cell transplantation and cellular therapy. , HLA, 89: 267-277
6. Kansagra AJ, Stefan MS (2016): Preoperative Anemia: Evaluation and Treatment. , Anesthesiol Clin., 34: 127-41
7. Christersson C, Johnell M, Siegbahn A. (2013): Evaluation of microparticles in whole blood by multicolour flow cytometry assay. , Scand J Clin Lab Invest., 73: 229-39
8. Grzyb T, Mrówczyńska L, Szczeszak A, Śniadecki Z, Runowski M, Idzikowski B, Lis S. (2015): Synthesis, characterization, and cytotoxicity in human erythrocytes of multifunctional, magnetic, and luminescent nanocrystalline rare earth fluorides. , J Nanopart Res., 17:399
9. Foucher B, Vila L. (2016): Bone marrow smear examination in the diagnosis of Niemann-Pick B disease. , Blood, 128: 738
10. Ząbczyk M, Sadowski M, Zalewski J, Undas A. (2015): Polyhedrocytes in intracoronary thrombi from patients with ST-elevation myocardial infarction. , Int J Cardiol., 179:186-7
11. Risso A, Ciana A, Achilli C, Antonutto G, Minetti G. (2014): Neocytolysis: none, one or many? A reappraisal and future perspectives. , Front Physiol., 5: 54
12. Viskupicova J, Blaskovic D, Galiniak S, Soszyński M, Bartosz G, Horakova L, Sadowska-Bartosz I. (2015): Effect of high glucose concentrations on human erythrocytes in vitro. , Redox Biol. , 5: 381-7
Nakład pracy studenta i punkty ECTS
| Forma aktywności | Średnia liczba godzin* na zrealizowanie aktywności | |
|---|---|---|
| Godziny zajęć (wg planu studiów) z nauczycielem | ||
| Praca własna studenta: | ||
| Przygotowanie do zajęć | ||
| Czytanie wskazanej literatury | ||
| Przygotowanie pracy pisemnej, raportu, prezentacji, demonstracji, itp. | ||
| Przygotowanie projektu | ||
| Przygotowanie pracy semestralnej | ||
| Przygotowanie do egzaminu / zaliczenia | ||
| SUMA GODZIN | ||
| LICZBA PUNKTÓW ECTS DLA MODUŁU ZAJĘĆ/PRZEDMIOTU | ||
* godzina (lekcyjna) oznacza 45 minut